Journal: Poultry Science

Article Title: Efficacy of a killed Salmonella Enterica serovar Typhimurium bacterin vaccine administration in layer birds challenged with heterologous Salmonella Enterica serovar Enteritidis

doi: 10.1016/j.psj.2025.105044

Figure Lengend Snippet: Effect of ST vaccine on cecal tonsil (5a) and spleen (5b) CD4+:CD8+ T cell ratios of vaccinated birds. Layer birds were randomly assigned to two groups, namely unvaccinated control and vaccinated groups. Birds were challenged with 5 × 10 8 CFU/mL of SE at 27 wk of age. At 1wk post-challenge on week 28, CD4+:CD8+ T cell percentage in the cecal tonsil and spleen were analyzed by flow cytometry (Guava Eascyte; Millipore). The result was reported as gated cell percentage and CD4+/CD8+ cell ratio was expressed. Means with no common superscript differ significantly ( P < 0.05). n = 8.

Article Snippet: Cells (1 × 10 6 ) were then incubated in a 96-well plate at 1: 200 dilution with PE-conjugated mouse anti-chicken CD4, 1: 200 dilution of FITC-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham AL, USA), or 1: 500 dilution of unlabeled mouse IgG for 20 minutes.

Techniques: Control, Flow Cytometry

Journal: Frontiers in Physiology

Article Title: A comparative evaluation of antibiotic and synbiotic supplementation on production performance and necrotic enteritis severity in broilers during an experimental necrotic enteritis challenge

doi: 10.3389/fphys.2024.1511380

Figure Lengend Snippet: Effect of synbiotic vs. antibiotic supplementation on intestinal permeability of broiler birds under an experimental NE challenge. Day-old chicks were distributed into four treatment groups. At days 21, 28, and 35, gut permeability was measured by serum FITC-d assay. Bars connected with star/s differ significantly (* P < 0.05 ; ** P < 0.01 ; *** P < 0.001 ; **** P < 0.0001 ). D21: P-value = 0.04; D28: P-value = 0.85; D35: P-value = 0.94 .

Article Snippet: Approximately 1 × 10 6 cells were mixed with FITC conjugated mouse anti-chicken CD4 cells (Catalog number 8210-90, Southern Biotech, Birmingham, AL) at a 1:250 dilution, or FITC conjugated mouse anti-chicken CD8 cells (Catalog number 8220-02, Southern Biotech) at a 1:450 dilution, along with unlabeled mouse IgG at a 1:100 dilution.

Techniques: Permeability

Journal: Frontiers in Physiology

Article Title: A comparative evaluation of antibiotic and synbiotic supplementation on production performance and necrotic enteritis severity in broilers during an experimental necrotic enteritis challenge

doi: 10.3389/fphys.2024.1511380

Figure Lengend Snippet: Effect of synbiotic vs. antibiotic supplementation on CD4+:CD8+ T cells in CT of broiler birds under an experimental NE challenge. Day-old chicks were distributed into four treatment groups. At days 21, 28, and 35, flowcytometry was performed to identify the percentage of CD4 + and CD8 + T cells in the cecal tonsil. Bars connected with star/s differ significantly (* P < 0.05 ; ** P < 0.01 ; *** P < 0.001 ; **** P < 0.0001 ). D21: P-value < 0.001; D28: P-value = 0.06; D35: P-value = 0.86 .

Article Snippet: Approximately 1 × 10 6 cells were mixed with FITC conjugated mouse anti-chicken CD4 cells (Catalog number 8210-90, Southern Biotech, Birmingham, AL) at a 1:250 dilution, or FITC conjugated mouse anti-chicken CD8 cells (Catalog number 8220-02, Southern Biotech) at a 1:450 dilution, along with unlabeled mouse IgG at a 1:100 dilution.

Techniques:

Journal: Frontiers in Physiology

Article Title: A comparative evaluation of antibiotic and synbiotic supplementation on production performance and necrotic enteritis severity in broilers during an experimental necrotic enteritis challenge

doi: 10.3389/fphys.2024.1511380

Figure Lengend Snippet: Effect of synbiotic vs. antibiotic supplementation on CD4+:CD8+ T cells in the spleen of broiler birds under an experimental NE challenge. Day-old chicks were distributed into four treatment groups. At days 21, 28, and 35, flowcytometry was performed to identify the percentage of CD4 + and CD8 + T cells in the spleen. Bars connected with star/s differ significantly (* P < 0.05 ; ** P < 0.01 ; *** P < 0.001 ; **** P < 0.0001 ). D21: P-value = 0.23; D28: P-value = 0.55; D35: P-value = 0.17.

Article Snippet: Approximately 1 × 10 6 cells were mixed with FITC conjugated mouse anti-chicken CD4 cells (Catalog number 8210-90, Southern Biotech, Birmingham, AL) at a 1:250 dilution, or FITC conjugated mouse anti-chicken CD8 cells (Catalog number 8220-02, Southern Biotech) at a 1:450 dilution, along with unlabeled mouse IgG at a 1:100 dilution.

Techniques: